International Journal of Technology Enhancements and Emerging Engineering Research (ISSN 2347-4289)

IJTEEE >> Volume 3 - Issue 8, August 2015 Edition

International Journal of Technology Enhancements and Emerging Engineering Research  
International Journal of Technology Enhancements and Emerging Engineering Research

Website: http://www.ijteee.org

ISSN 2347-4289

None-Alcoholic Beverages Formulated From Selected Traditional Sudanese Fruits Fermented With Bifdobacterium Infantis 20088

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Wala Salah Elden, Abubakar Awad Siddig, Barka Mohammed Kabeir



Keywords: Sudanese, Gudaim, Dom, Fermentation, Probiotics, Bifidobacterium.



Abstract: This study was carried out to develop none-alcoholic beverage from traditional Sudanese fruits dom (Hyphaene thebaica) and gudaim (Grewia tenax) using Bifidobacterium infantis 20088. The fruits were cleaned by removing the dirt and foreign materials. The seeds were separated from fruits except for gudaim fruit was directly used. The growth medium was formulated from10% (w/v) reconstituted skim milk (the control); in addition to 7.5% (w/v) dom and gudaim supplemented with 2.5 g skim milk to provide the required nutrient for bacteria growth during the fermentation.. After sterilization of growth medium and cooling, the mixture was inoculated with a 10% culture of B.Infants followed by incubation for 60 h at 37 ◦C. The results obtained revealed highest growth of B. infantis in gudaim beverage, followed by reconstituted skim milk, and then fermented dom. That is because gudaim contained the highest level of glucose as compared to skim milk and dom fruit. In general there was significant (p < 0.05) increase in maximum viable count of each formula as compared with its initial level at the beginning of fermentation in al beverages. The increased viable number was accompanied by a significant p < 0.05) reduction in pH and total soluble solids (TSS). Hopefully the strain viable counts fulfill the number required to presence in probiotic foods, which at least 1106 CFU/ml fermented product. Therefore gudaim and dom are suitable carrier for Bifid bacterium infantis.



[1] Salovaara, H. (1999). Functional food: a global perspective .Cereal food world 44: 98.

[2] Fuller, R., Gibson, G.R. (1997). Modification of the intestinal microflora using probiotics and prebiotics.Scand. Journal of Gastoenterology 222: 28S-31S.

[3] Metchnikoff, E. (1907). The Prolongation of life: Optimistic studies. Heinemann, London.

[4] Schrezenmeir, J., de Vrese, M. (2001). Probiotics, Prebiotics, and synbiotics approaching adefinition. American Journal of Clinical Nutrition 73: 361S-364 S.

[5] Tomasik, P.J., Tomasik, P. (2003). Probiotics and prebiotics. Cereal Chemistry 80: 113 117.

[6] FAO/WHO. (2001). Reports of a joint FAO/WHO expert consultation on evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria, American Cordoba Park Hotel, Cordoba, Argentina, 1 4 October 2001.

[7] Wang, Y., Yu, R., Chou, C. (2002). Growth and survival of bifidobacteria and lactic acid bacteria during the fermentation and storage of cultured soymilk dirinks. Food Microbiology 19: 501 - 508.

[8] Kabeir B.M.,Abd Aziz, S,Muhammad, M,and Yazid, A.M. (2005). Growth of Bifidobacterium longum BB36 in Medida (fermented cereal porridge) and their survival during storage.lett.AppI. Microbiol. 41: 12-131.

[9] AOAC. (1990). Official Methods for Analysis(15th ed) . Association of Official Analytical Chemists. Washington, D.C., USA.

[10] MINITAB. (2006). Statistical software, Release 14 for Windows, minitab Inc, USA.

[11] Desjardins, M.L.,Roy, D., Goulet, J. (1990). Growth of bifdobacteia and their enzyme profile. Journal of Dairy Science, 73: 299-307.

[12] Dubey U.K, Mistry V.V. (1996). Effect of Bifidogenic Factor on Growth Characteristic of Bifidobacteia in infant formula. Pp. 116-1163.

[13] Chou, C.C and Hou, J.W. (2000). Growth of bifidobacteria in soymilk and their survival in the fermented drink during storage. Int J Food Microbial., 6: 113-121.

[14] Lankaputhra, W.E.V., Shah, N.P., Bri Z, M.L. (1996). Survival of bifidobacteria during refrigerated storage in the presence of acid and hydrogen peroxide. Milchwissenschaft, 51: 65-70.

[15] Bullen, C.L., Tearle, P.V., Willis, A.T. (1976). Bifidobacteria in the intestinal tract of infants: as in-vivo study. J Med Microbial., 9: 325- 333.