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International Journal of Technology Enhancements and Emerging Engineering Research (ISSN 2347-4289)

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International Journal of Technology Enhancements and Emerging Engineering Research  
International Journal of Technology Enhancements and Emerging Engineering Research

Website: http://www.ijteee.org

ISSN 2347-4289

Formulation And Evaluation Microcapsules Of Caesalpinia Sappan Linn. Using Emulsion Solvent Evaporation Method

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Andi Dian Permana, Rifka Nurul Utami, Awalyah Ramadhani, Musfira Dewy, Bobby Sugara



Keywords: Caesalpinia sappan, Microcapsule, Solvent evaporation, Antioxidant



ABSTRACT: Caesalpinia extract has widely investigated as a potent source of antioxidants. Its antioxidant activity is majorly due to its high level of phenolic compounds. Microencapsulation is one of the stabilization methods existing to overcome the instability of polyphenol in order to maintain its stability prior to administration. The objective of this research is to encapsulate the caesalpinia extract using emulsion solvent removal method using ethyl cellulose as encapsulating agent and evaluate the physical characteristic and dissolution rate of encapsulated material. The heart wood of caesalpinia plant was extracted using three different solvent: 70% ethanol, 96% ethanol and destilled water. The three extracts obtained from maceration then measured its antioxidant activity and total phenolic compounds (TPC). The 70% ethanol extract shows the greatest TPC (48.36 %w/w) and IC50 in DPPH (3.572 ppm), thus, this extract was selected to be encapsulated. Microcapsules were prepared with three different drug-polymer ratio used in the formulas, 1 : 3, 1 : 4 and 1 : 5. These formulas were evaluated for drug encapsulation efficiency, microcapsule wall thickness and morphology, and also in vitro drug dissolution rate. According to the results obtained in this study, the microcapsule with the greatest physical characteristic and dissolution rate that is suitable for sustained release administration is formula III. From this study, it is suggested that caesalpinia extract is able to be formulated into microcapsules using emulsion solvent evaporation method and futher developed into sustained release oral preparation as a source of antioxidants.



[1] B.I. Utomo,“CaesalpiniaL.” Plant resources of South-East Asia. Medicinal and poisonous plants II, J.L.C. H. van Valkenburg and N. Bunyapraphatsara, eds., Bogor: Prosea Foundation, pp. 123-129, 2002.

[2] S. Badami, S. Moorkoth, B. Suresh, “Caesalpiniasappan: A Medicinal and Dye Yielding Plant”. J. Natural Product Radiance, vol. 3 no. 2, 2004.

[3] S. R. Oh, D.S. Kim, I.S. Lee, K.Y. Jung, J.J. Lee, H.K. Lee, “Anticomplementary activity of constituents from the heartwood of Caesalpiniasappan”, J. Planta Med, vol. 64, pp. 456-458, 1998.

[4] M.Y. Lim, J.H. Jeon, E.Y. Jeong, C.H. Lee, H.S. Lee, “Antimicrobial activity of 5-hydroxy-1,4-naphthoquinone isolated from Caesalpiniasappan toward intestinal bacteria”, J. Food Chem, vol. 100, pp. 1254-1258, 2007.

[5] S. Badami, S. Moorkoth, S.R. Rai, E. Kannan, S. Bhojraj, “Antioxidant activity of Caesalpiniasappan heartwood”, Biol Pharm Bull, vol. 26, pp. 1534-1537, 2003.

[6] J. Javanmardi, C. Stushnoff, E. Locke, J.M. Vivanco, “Antioxidant activity and total phenolics content of Iranian ocimum accessions”, J. Food Chem, vol. 83, pp. 547-550, 2003.

[7] P. Wetwitayaklung, T. Phaechamud, S. Keokitichai, “The antioxidant activity of Caesalpinasappan L. heartwood in various ages”, J. Naresuan University, vol. 13 no. 2, pp. 43-45, 2005.

[8] C. Saenjum, C. Chaiyasut, S. Kadchumsang, S. Chansakaow, M. Suttajit, “Antioxidant activity and protective effects on DNA damage of Caesalpiniasappan L. extract” J. Medicinal Pla Res, vol. 4 no. 15, pp. 1594-1600, 2010.

[9] S.H. Benabadji, R. Wen, J.B. Zheng, X.C. Dong, S.G. Yuan, “Anticarcinogenic and antioxidant activity of di indolylmeethanederavatives”, J. ActapharmacolSci, vol.25, pp. 666-667, 2004.

[10] N.I. Baek, S.G. Jeon, E.M. Ahn, J.T. Hahn, J.H. Bahn, S.W. Cho, “Anticonvulsant compounds from the wood of Caesalpiniasappan L.”, J. Arch Pharm Res, vol. 23, pp. 344-348, 2002.

[11] K. Sarumathy, T. Vijay, S. Palani, K. Sakthivel, M.S. DhanaRajan, “Antioxidant and hepatoprotective effects of Caesalpiniasappan against acetaminophen-induced hepatotoxicity in rats”, Int. J. PharmacolTherape, vol. 1, pp. 19-31, 2011.

[12] H.X. Xu, S.F. Lee,“The antibacterial principle of Caesalpiniasappan”, J. Phytother Res, vol. 18, pp. 647-651, 2004.

[13] V. S. Srilakshmi,P. Vijayan, P.V. Raj, S.A.Dhanaraj, H. R. Chandrashekhar,“Hepatoprotective properties of Caesalpinasappan Linn. Heartwood on carbon tetrachloride induced toxicity”,Ind. J. Exe Biol, vol. 48, pp. 905-910, 2010.

[14] M. Namikoshi, H. Nakata, T.Saitoh,“Homoisoflavonoids and related compounds: V. A novel dibenzoxocin derivative from Caesalpiniasappan L.”Chemical and Pharmaceutical Bulletin, vol. 35, pp. 3615-3619, 1987.

[15] M. Namikoshi, H. Nakata, H. Yamada, M. Nagai, T.Saitoh, “Homoisoflavonoids and related compounds: II. Isolation and absolute configurations of 3,4-dihydroxylated homoisoflavans and brazilins from Caesalpiniasappan L.” Chemical and Pharmaceutical Bulletin, vol. 35, pp. 2761-2773, 1987.

[16] M. Nagai andS. Nagumo,“Protosappanin B, a new dibenzoxocin derivative from sappan lignum (Caesalpiniasappan)”J.Heterocycles, vol. 24, pp. 601-606, 1986.

[17] M. Nagai, S.Nagumo, S. M. Lee, I. Eguchi,K. I. Ka-wai,“Protosappanin A, a novel biphenyl compound from sappan lignum” Chemical and Pharmaceutical Bulletin, vol. 34, pp. 1-6,1986.

[18] C. Fuke, J.Yamahara, T.Shimokawa, J. Kinjo, T.Tomimatsu, T. Nohara, “Two aromatic compounds related to brazilin from Caesalpiniasappan”J. Phytochemistry, vol. 24, pp. 2403-2406, 1985.

[19] D.S. Kim, N.I. Baek, S. R. Oh, K.Y. Jung, I. S. Lee, H.K. Lee, “NMR assignment of brazilin”, J.Phytochemistry, vol. 46, pp. 177-178, 1997.

[20] K. Miyahara, T. Kawasaki, J. E.Kinojo, T.Shimokawa, J. Yamahara, M. Yamasaki, “The X-ray analysis ofcaesalpin J from sappan lignum”, Chemical and Pharmaceutical Bulletin, vol. 34, pp. 4166-4169,1986.

[21] C. K. Moon,et al.,“Effects of brazilin on erythrocyte deformability and its related biochemical factors in streptozotocin induced diabetic rats”,Archives of Pharmacal Research, vol. 11, pp. 149-154, 1988.

[22] S. Y. Choi,et al.,“Brazilin modulates immune function mainly by augmenting T cell activity in halothane administered mice” J.PlantaMedica, vol. 63, pp. 405-408, 1997.

[23] S. Badami, S.Moorkoth, S. R.Rai, E.Kannan, S.Bhojraj, “Antioxidant activity of Caesalpiniasappan heartwood”, Biological and Pharmaceutical Bulletin, vol. 26, pp. 1534-1537, available at https://www.jstage.jst.go.jp/ ar-ticle/bpb/26/11/26_11_1534/_article, 2003.

[24] N. I. Baek,et al.,“Anticonvulsant compounds from the wood of Caesalpiniasappan L.”,Archives of Pharmacal Research, vol. 23, pp. 344-348, 2000.

[25] T. Saitoh, S.Sakashita, H. Nakata, T.Shimokawa, K. Kinjo, J.Yamahara, “3-Benzylchroman derivatives related to brazilin from sappan lignum”,Chemical and Pharmaceutical Bulletin, vol. 34, pp. 2506-2511, 1986.

[26] P. Yingming, L. Ying, W.Hengshan, L. Min, “Antioxi-dant activities of several Chinese medicine herbs” J. Food Chemistry, vol. 88, pp. 347-350, available at http://www.sciencedirect.com/science/article/pii/S0308814604001190,2004.

[27] T. F. Vandamme, D.Poncelet, P.Subra-Paternault, Microencapsulation: des sciences aux technologies, Paris: Lavoisier Tec, 2007.

[28] R. Arshady, Microspheres Microcapsules & Lipo-somes: Preparation & Chemical Applications, Lon-don:Citus Books, 1999.

[29] S. Benita, Microencapsulation: Methods and Industrial Applications; Boca Raton, Flo.: Taylor & Francis, 2006.

[30] C. N. Shanthi, R. Gupta, A.K.Mahato, “Traditional and emerging applications of microspheres: A review”, Int. J. PharmTech Res, vol. 2, pp. 675-681, 2010.

[31] H. M. C. Azeredo, “Encapsulação: aplicação à tecnologia de alimentos”, J.Alimentos e Nutrição, vol. 16 no. 1, pp. 89-97, 2005.

[32] P. Trivedi,A. Verma, N.Garud,“Preparation and cha-racterization of aceclofenac microspheres”,Asian J. Pharm., vol. 2 no. 2, pp. 110, 2008.

[33] H. Wantier, F. Mathieu, M.Baudrihaye, D. Dela-croix,“Microspheres for the controlled release of water-soluble substances and process for preparing them”,Google Patents, 1997.

[34] S.Y. Lin, K. H. Lin, M. J. Li,“Formulation design of double-layer in the outer shell of dry-coated tablet to modulate lag time and timecontrolled dissolution function: studies on micronized ethylcellulose for dosage form design (VII)”, J. AAPS, vol. 6 no. 3, pp. e17, 2004.

[35] Soskolne, G.Golomb, M. Friedman, M.N.Sela,“New sustained release dosage form of chlorhexidine for dental use .II. Use in periodontal therapy”,J. Periodontal Res., vol. 18 no. 3, pp. 330-336, 1983.

[36] D. L. Sundari,Widowati, M.W. Winarno, “InformasiKhasiat, KeamanandanFitokimiaTanamanSe-cang(CaesalpiniasappanL.)”,Warta TumbuhanObat Indonesia, 1998.

[37] J. Sri, A. Seethadevi, K. S. Prabha, P. Muthuprasanna, P. Pavitra, “Microencapsulation : A Review”, Int. J. Pharm Bio Sci, vol. 13 no. 1, pp. 509-531, 2012.

[38] S. Purwaningsih, “Aktivitas Antioksidan dan Komposisi Kimia Keong Mata Merah (Cerithidea obtusa)”, J. Ilmu Kelautan, vol. 17 no. 1, pp. 42, 2012.

[39] M. N. Singh, K. S. Y. Hemant, M. Ram, H. G. Shivakumar, “Microencapsulation: A Promising Technique for Controlled Drug Delivery”, J. Res Pharm Sci, vol. 5 no. 2, pp. 65-77, available at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093624/, 2010.