IJTEEE
International Journal of Technology Enhancements and Emerging Engineering Research (ISSN 2347-4289)
PREVIOUS PUBLICATIONS



IJTEEE >> Volume 2 - Issue 7, July 2014 Edition



International Journal of Technology Enhancements and Emerging Engineering Research  
International Journal of Technology Enhancements and Emerging Engineering Research

Website: http://www.ijteee.org

ISSN 2347-4289



Real Time And Traditional Polymerase Chain Reaction: A Comparative Study

[Full Text]

 

AUTHOR(S)

Simran Singh, Saurabh Rawat, Okesh Chabbra

 

KEYWORDS

Index Terms: Formulation, Zone of Inhibition, Antibiotic Sensitivity Test.

 

ABSTRACT

Abstract: Real-Time PCR—also called quantitative polymerase chain reaction (qPCR)—is one of the most powerful and sensitive gene analysis techniques available and is used for a broad range of applications including quantitative gene expression analysis, genotyping, SNP analysis, pathogen detection, drug target validation and for measuring RNA interference. Frequently, real-time polymerase chain reaction is combined with reverse transcription to quantify messenger RNA (mRNA) and MicroRNA (miRNA) in cells or tissues. Traditional methods use Agarose gels for detection of PCR amplification at the final phase or end-point of the PCR reaction. To understand why traditional PCR is limiting, it is important to understand what happens during a PCR reaction.

 

REFERENCES

[1] http://www6.appliedbiosystems.com/support/tutorials/pdf/rtpcr_vs_tradpcr.pdf

[2] http://www3.appliedbiosystems.com/cms/groups/mcb_marketing/documents/generaldocuments/cms_042485.pdf

[3] http://www.eurogentec.com/uploads/qPCR-guide.pdf

[4] http://www.viracoribt.com/Learning-Lab/PCR

[5] pathmicro.med.sc.edu/pcr/realtime-home.htm

[6] www.lifetechnologies.com/in/en/home/life.../pcr/real-time-pcr.html

[7] www.bio.davidson.edu/courses/molbio/molstudents/.../realtimepcr.htm

[8] qpcr.gene-quantification.info/