IJTEEE
International Journal of Technology Enhancements and Emerging Engineering Research (ISSN 2347-4289)
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IJTEEE >> Volume 3 - Issue 6, June 2015 Edition



International Journal of Technology Enhancements and Emerging Engineering Research  
International Journal of Technology Enhancements and Emerging Engineering Research

Website: http://www.ijteee.org

ISSN 2347-4289



Separation Of Leaves Pigment In Case Of “Endod” Leaves By Using Thin Layer Chromatography (TLC)

[Full Text]

 

AUTHOR(S)

Teklay Mezgebe, Getu Shura

 

KEYWORDS

Keywords: Chromatography, Thin layer chromatography, Retention factor, Mobile phase, Stationary phase.

 

ABSTRACT

Abstract: Chromatography is one of the analytical methods that able to separate components of the plant leaves sample based on their interaction between stationary phase and mobile phase. Thin layer chromatography has valuable role to separate pigments in leaves based on the rate of reaction with mobile phase. The ratio of RF values are 0.73, 0.93, 0.55, and 0.35 for blue green, golden, yellow and yellow green colour respectively. The ratio RF values occurred between 0 and1, the largest ratio the better resolution, while the smallest ratio the poor resolution .

 

REFERENCES

[1] Dougles A. skoog, Donald M. west an introduction to chromatographic method, pp 660, standford university.

[2] Gary D, Christian, (1971), chromatography principles and theory,pp-555, Russia .

[3] L.S. Ettre, (1993), “Nomenclature for chromatography” pure and applied chemistry, pp 1819- 72, Russia.

[4] B, Fried and J. sherma, (1999), thin layer chromatography 4thed, pp65 – 70, New York.