In-Vitro Evaluation And Testing For The Antagonistic Activity Of Trichoderma Isolates Against Coffee Wilt Disease (Fusarium Xylarioides)
Key Words: Coffee wilt disease, Fusariumxylarioides, Trichoderma, Dual culture, antagonistic test.
ABSTRACT: Coffee arabicais the key cash crop and top mainstay of the Ethiopian economy and requires sustainable production methods.Coffee wilt disease is one of the reasons for the reduction in coffee productivity as well in the livelihoods of millions involved in cultivation, processing, marketing, and export of coffee. The major causative agent of coffee wilt disease is Fusariumxylarioides. Trichodermaspecies are known for the effective control for this particular pathogen. In the present study;Trichoderma species were isolated from the soil sample taken from Jimma and the antagonistic efficiency of Trichoderma isolates against Fusariumxylarioideswas confirmed, using dual culture method. Trichodermaisolates significantly inhibited the mycelial growth of Fusariumxylarioidesranging from 83.08% - 81.14%, after 6 to 10 days of incubation at 25 o. Trichoderma isolates showed successful antagonism against the test pathogen (Fusariumxylarioides).
 Bayetta, B. (2001). Arabica coffee breeding for yield and resistance to coffee berry disease (Colletotrichumkahawae sp. nov.). Dissertation, University of London, Imperial College Wye, U. K.
 Bissett, J. (1991). A revision of the genus Trichoderma.II.Infrageneric classification.Can. J. Bot.69: 2357-2372.
 Fernie L. M. (1966). Some impressions of coffee in Ethiopia. Kenya Coffee 31: 115-121.
 Fraselle J. (1950). Observations préliminairessurunetrachéomycosede Coffearobusta. Bull. Agricole du Congo Belge, 41: 361-372.
 Harman, E. G., Howell, R. C., Viterbo, A., Chet, I. and Matteo, L.(2004). Trichoderma species opportunistic, avirulent plant symbionts.Nature Rev. Mic. 2: 43-56.
 Mekuria, T., Neuhoff, D. and Köpke, U. (2004). The status of coffee production and the potential for organic conversion in Ethiopia. Conference on International Agricultural Research for Development, Berlin, October 5-7.
 NegashHailu (2007). Isolation, Identification of Fusariumxylarioidesfrom Southern Ethiopia and its response to Fungal Biocontrol Agents.Msc Thesis, Addis Ababa University.
 Perello, A., Monaco, C., Simond, M., Sisterna, M. and Bello, G. (2003). Biocontrol efficacy of Trichodermaisolates for tan spot of wheat in Argentina. Crop Prot. 22:1099–1106.
 Phiri N. and Baker, P.S. (2009) Coffee Wilt in Africa Final Technical Report. CAB International.
 Rutherford, A. M., 2006. Current knowledge of coffee wilt disease, a major constraint to coffee production in Africa.The American Phytopathol.Soci.6:663-666.
 Susanto, A., Sudharto, P. and Purba, R. (2005). Enhancing biological control of basal stem rot disease (Ganodermaboninense) in oil palm plantations. Mycopathol.159:153–157.
 TemesgenBelayneh, Christian, P. K. and Irina, S. D. (2010). The rhizosphere of Coffee arabicain its native highland forests of Ethiopia provides a niche for a distinguished diversity of Trichoderma. Diversity 2: 527-549.
 UN, (2004). Human Development Report.Economica, Paris.
 YonasUrbanos (2010).Characterization of antagonistic Trichodermaspecies against coffee wilt disease(Fusariumxylarioides). Msc Thesis, Addis Ababa University.