Open Access Journal of Scientific, Technology & Engineering Research

International Journal of Technology Enhancements and Emerging Engineering Research (ISSN 2347-4289)

IJTEEE >> Volume 3 - Issue 5, May 2015 Edition

International Journal of Technology Enhancements and Emerging Engineering Research  
International Journal of Technology Enhancements and Emerging Engineering Research

Website: http://www.ijteee.org

ISSN 2347-4289

Mycogenic Silver Nanoparticle Biosynthesis And Its Pesticide Degradation Potentials

[Full Text]



Alak Chandra Deka, Swapan Kumar Sinha



Keywords : Penicillium pinophilum, extracellular fungal secrete, Silver nanoparticles, pesticide degradation, Chlorpyrifos, FTIR, XRD



ABSTRACT: Chemical pesticides are resistant to biodegradation beside carcinogenic in nature even at trace levels. Effort to remove pesticide by conventional methods has several inherent disadvantages of longer time or cost and this bottleneck can be avoided by utiliz-ing nanoparticles as it can mineralise at a faster rate. Nanoparticles are syntheised by reduction of metal ions using physical, chemical and biological process either alone or in combination. In the present study biosynthesis of silver nanoparticle (AgNPs) using hydrophilic fungal secretes (HFS) of Penicillium pinophilum and degradation of chlorpyrifos pesticides in different pH environment has been reported. The AgNPs formed were characterized by using UV-Visible spectrum, FTIR and XRD. The HFS is the supernant of 72h incubated 10 g of mycelial mat in 100 ml deionized water at 28°C. AgNPs were synthesized by the reaction of HFS with 1 mM silver nitrate solution at 1:5 ratio. The spectrophotometric absorption peak at 420 nm is due to the Surface Plasmon response (SPR) properties of AgNPs. FTIR anlysis of HFS revealed functional groups are 1096 cm-1 (C-O & C-C Stretch), 1664 cm-1 (C=C Stretch), 2907 cm-1 (C-H Stretch), 2931 cm-1 (C-H Stretch), 3475 cm-1 (O-H, N-H Stretch). The outranking of functional groups in Ag+ reduction using depletion percent of FTIR bands before and after reaction revealed the O-H (Alcohols, phenols) and C-H (Alkenes) functional groups showed highest (>40%) whereas functional group esters were the lowest (<10%). AgNPs was examined by the XRD pattern of diffraction peaks showed at 2θ values of 32.23°, 46.18°, 64.82° and 77.21° assigned to the planes of (111), (200),(220) and (311) faced centre cubic (fcc) of silver nanoparticles. The chlorpyrifos degradation by AgNPs showed highest in mild acidic (pH-6) followed by alkaline (pH 9) and astrong acidic (pH 0.3) environment.



[1] C. J. Wang and Z.Q. Liu, “Foliar uptake of pesticides-Present status and future challenge Pesticide,” Journal of Biochemistry and Physiology, 87:1–8, 2007.

[2] J. Liu, S.Z. Qiao, Q.H. Hu, and G.Q. Lu, “Magnetic nanocompo-sites with mesoporous structures: synthesis and applications,” Small, 7(4):425–443, 2011.

[3] N.A. Luechinger, R.N. Grass, E.K.Athanassiou, and W.J. Stark, “Bottom-up fabrication of metal/metal nanocomposites from na-noparticles of immiscible metals,” Chemistry of Materials, 22(1): 155–160, 2010.

[4] V. Bansal, R. Ramanathan and S.K. Bhargava, “Fungus-mediated biological approaches towards green synthesis of oxide nanomaterials”. Aust. J. Chem. 64: 279 293, 2011.

[5] M. Kowshik, N. Deshmukh, W. Vogel, J. Urban, S.K. Kulkarni, and K.M. Paknikar, “Microbial synthesis of semiconductor CdS nanoparticles, their characterization, and their use in the fabrication of an ideal diode”. Biotechnology and Bioengineering, 78(5): 583-588, 2002.

[6] A. Cauerhff and G.R. Castro, “Bionanoparticles, a green nano-chemistry approach. ” Electronic Journal of Biotechnology ISSN: 0717-3458 , 2013, 16 (3), Issue of May 15, 2013

[7] P . Mukherjee, M. Roy, B.P. Mandal , G.K. Dey, P.K. Mukherjee, J. Ghatak, A. K. Tyagi and S.P. Kale , “Green synthesis of highly stabilized nanocrystalline silver particles by a non-pathogenic and agriculturally important fungus T. asperellum”, Nanotech-nology 2008; 19 075103. doi:10.1088/0957-4484/19/7/075103

[8] N.A. Jain, S. Bhargava, J.C. Majumdar,Tarafdar, J. Panwar, “Extracellular biosynthesis and characterization of silver nano-particles using Aspergillus flavus NJP08: A mechanism perspec-tive”. Nanoscale, 3: 635-641, 2010

[9] U. Kumar, A.K. Ranjan, C. Sharan, A.A. Hardikarc, A. Pundle, P. Poddar, “ Green approach towards size controlled synthesis of biocompatible antibacterial metal nanoparticles in aqueous phase using lysozyme”. Curr Nanosci. 8:130–140, 2012.

[10] D.S. Balaji, S. Basavaraja, R. Deshpande, D.B. Mahesh, B.K. Prabhakar, A.Venkataraman, “Extracellular biosynthesis of func-tionalized silver nanoparticles by strains of Cladosporium cla-dosporioides fungus”. Colloids and Surfaces B: Biointerfaces, 68: 88-92, 2009.

[11] S. Honary, H. Barabadi, E. Gharaei-Fathabad and F.Naghibi , “Green Synthesis of Silver Nanoparticles Induced by the Fungus Penicillium citrinum”. Tropical Journal of Pharmaceutical Research February 12 (1): 7-11, 2013.

[12] S.K. Vanmathi, T. Sivakumar, Isolation and characterization of silver nanoparticles from Fusarium oxysporum. International Journal of Current, Microbiology and Applied Science, 1(1):56-62, 2012.

[13] A. Ahmad, P. Mukherjee, S. Senapati, D. Mandal,, M.I. Khan, R. Kumar, and M. Sastry , “Extracellular biosynthesis of silver na-noparticles using the fungus Fusarium oxysporum”. Colloids surf.B, 28: 313, 2003.

[14] K.S. Naveen, G. Kumar, L. Karthik and K.V. Rao, “Extracellular biosynthesis of silver nanoparticles using the Filamentous Fungus Penicillium Sp”, Archives of Applied Science Research, 2(6) : 161-167, 2010.

[15] A. Ingle, A. Gade, S. Pierrat, C. Sonnichsen and M. Rai, “My-cosynthesis of silver nanoparticles using the fungus Fusarium acuminatum and its activity against some human pathogenic bacteria”.Curr. Nanosci, 4: 141-144, 2008.

[16] F. Rahman, S. Deshmukh, A. Ingle, A. Gade, M. Rai, “Silver nanoparticles: Novel antimicrobial agent synthesized from an endophytic fungus Pestalotia sp. isolated from leaves of Syzy-gium cumini ”, Nano Biomed Eng, 3 (3):174–178, 2011

[17] V.C.Verma, R.V. Kharwar, A.C. Gange, “Biosyntheis of antimi-crobial silver nanoparticles by the endophytic fungus Aspergillus clavatus ”. Nanomedicine, 5(1):33-40, 2010.

[18] J. Mussarat, S. Dwivedi, B.R. Sinh, A.A. Al-Khedhariya, A. Azam, A. Naqvi, “Production of antimicrobial silver nanoparticles in water extracts of fungus Amylomyces rouxii strain KSU-09”, Bioresource Technol, 101: 8772–8776, 2010.

[19] Bharathidasan R., Panneerselvam A., “Biosynthesis and charac-terization of silver nanoparticles using endophytic fungi Aspergillus concius, Penicillium janthinellum and Phomosis sp”. International Journal of Pharmaceutical Science and Research, 3(9): 3163-3169, 2012.

[20] L. Kvishek and R. Prucek , “The preparation and application of silver nanoparticles”. Journal of Materials Science, 22: 2461-2473, 2005.

[21] L. F. Johnson, and E.A. Curl, “Methods for Research on the Ecology of Soil- Borne Pathogens”. Burgress Publ. Co. Minne-apolis, Minnesota. pp: 247, 1972

[22] A.V.B. Reddy, V. Madhavi, K.G. Reddy, and G. Madhavi, “Re-mediation of Chlorpyrifos-Contaminated Soils by Laboratory-Synthesized Zero-Valent Nano Iron Particles: Effect of pH and Aluminium Salts” Journal of Chemistry, Hindawi Publishing Cor-poration Volume 2013, Article ID 521045, 7 pages, 2013.

[23] T. Pradeep, Anushup, Thin solid films 517: 6441, 2009.

[24] K.V. Selvi and T. Sivakumar, “Isolation and characterization of silver nanoparticles from Fusarium oxysporum”. Int.J.Curr.Microbiol. App.Sci. 1(1):56-62, 2012.

[25] A.Kadir, R. Joseph, Lakowiez and C.D. Geddes. Rapid deposi-tion of triangular silver nanoplates on planar surfaces: application to metal-enhanced fluorescence. J. Phys. Chem. B. 109, 6247-6251. 2005.

[26] Dattu Singh, Vandana Rathod, Shivaraj Ninganagouda, Jyothi Hiremath, Ashish Kumar Singh, and Jasmine Mathew. Optimi-zation and Characterization of Silver Nanoparticle by Endophytic Fungi Penicillium sp. Isolated from Curcuma longa (Turmeric) and Application Studies against MDR E. coli and S. aureus Volume 2014 (2014), Article ID 408021, 2014

[27] A. Banu, V. Rathod, and E. Ranganath, “Silver nanoparticle pro-duction by Rhizopus stolonifer and its antibacterial activity against extended spectrum β-lactamase producing (ESBL) strains of Enterobacteriaceae,” Materials Research Bulletin, vol. 46, no. 9, pp. 1417–1423, 2011.

[28] Sukdeb Pal, Yu Kyung Tak, and Joon Myong Song, Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli, Appl. Environ. Microbiol. 73 : 1712-1720, 2007.

[29] Laura Obreja, N. Foca , M. I. Popa , V. Melnig , Alcoholic reduction platinum nanoparticles synthesis by sonochemical methods. Scientific Annals of “Alexandru Iron Cuza Din Iasi” University Tomul I, s. Biomaterials in Biophysics, Medical Physics and Ecology , 2008.

[30] G. Alagumuthu, R. Kirubha, Synthesis and Characterisation of Silver Nanoparticles in Different Medium. Open Journal of Syn-thesis Theory and Applications, 1, 13-17, 2012.

[31] B. Deepa and V. Ganesan, Bioinspiredsynthesis of selenium nanoparticles using flowers of Catharanthus roseus(L.) G. Don. and Peltophorum pterocarpum(DC.)Backer ex Heyne – a com-parison. Int.J. ChemTech Res.2014-2015,7(2),pp 725-733, 2015.

[32] R. Varshney, A.N. Mishra, S. Bhadauria, M.S. Gaur, Digest Journal of Nanomaterials and Biostructures, 4(2), 349 – 355, 2009.

[33] M. Amutha, P. Lalitha, M. J. Firdhouse, “ Biosynthesis of Silver Nanoparticles Using Kedrostis foetidissima (Jacq.) Cogn”. Journal of Nanotechnology Volume 2014 (2014), Article ID 860875, 5 pages, http://dx.doi.org/10.1155/2014/860875.