Mycogenic Silver Nanoparticle Biosynthesis And Its Pesticide Degradation Potentials
Alak Chandra Deka, Swapan Kumar Sinha
Keywords : Penicillium pinophilum, extracellular fungal secrete, Silver nanoparticles, pesticide degradation, Chlorpyrifos, FTIR, XRD
ABSTRACT: Chemical pesticides are resistant to biodegradation beside carcinogenic in nature even at trace levels. Effort to remove pesticide by conventional methods has several inherent disadvantages of longer time or cost and this bottleneck can be avoided by utiliz-ing nanoparticles as it can mineralise at a faster rate. Nanoparticles are syntheised by reduction of metal ions using physical, chemical and biological process either alone or in combination. In the present study biosynthesis of silver nanoparticle (AgNPs) using hydrophilic fungal secretes (HFS) of Penicillium pinophilum and degradation of chlorpyrifos pesticides in different pH environment has been reported. The AgNPs formed were characterized by using UV-Visible spectrum, FTIR and XRD. The HFS is the supernant of 72h incubated 10 g of mycelial mat in 100 ml deionized water at 28°C. AgNPs were synthesized by the reaction of HFS with 1 mM silver nitrate solution at 1:5 ratio. The spectrophotometric absorption peak at 420 nm is due to the Surface Plasmon response (SPR) properties of AgNPs. FTIR anlysis of HFS revealed functional groups are 1096 cm-1 (C-O & C-C Stretch), 1664 cm-1 (C=C Stretch), 2907 cm-1 (C-H Stretch), 2931 cm-1 (C-H Stretch), 3475 cm-1 (O-H, N-H Stretch). The outranking of functional groups in Ag+ reduction using depletion percent of FTIR bands before and after reaction revealed the O-H (Alcohols, phenols) and C-H (Alkenes) functional groups showed highest (>40%) whereas functional group esters were the lowest (<10%). AgNPs was examined by the XRD pattern of diffraction peaks showed at 2θ values of 32.23°, 46.18°, 64.82° and 77.21° assigned to the planes of (111), (200),(220) and (311) faced centre cubic (fcc) of silver nanoparticles. The chlorpyrifos degradation by AgNPs showed highest in mild acidic (pH-6) followed by alkaline (pH 9) and astrong acidic (pH 0.3) environment.
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